Prof. Jingtao Fan, 

Tsinghua University,Beijing,China.

Presentation title: Iterative tomography with digital adaptive optics permits hour-long intravital observation of 3D subcellular dynamics at millisecond scale

Abstract: Long-term subcellular intravital imaging in mammals is vital to study diverse intercellular behaviors and organelle functions during native physiological processes. However, optical heterogeneity, tissue opacity, and phototoxicity pose great challenges. Here, we propose a computational imaging framework, termed digital adaptive optics scanning light-field mutual iterative tomography (DAOSLIMIT), featuring high-speed, high-resolution 3D imaging, tiled wavefront correction, and low phototoxicity with a compact system. By tomographic imaging of the entire volume simultaneously, we obtained volumetric imaging across 225× 225×16 mm3, with a resolution of up to 220 nm laterally and 400 nm axially, at the millisecond scale, over hundreds of thousands of time points. To establish the capabilities, we investigated large-scale cell migration and neural activities in different species and observed various subcellular dynamics in mammals during neutrophil migration and tumor cell circulation. 

Biography:

Jingtao Fan received the B.E. and M.E. degrees in computer science and technology and the Ph.D. degree in optical engineering from the Changchun University of Science and Technology, Changchun, China, in 2003, 2007, and 2013, respectively. He currently is a research track associate professor of Tsinghua University, Beijing, China. His current research interests include cognitive sciences and mesoscale microscopy.